Pancreatic cancer (PC) is a global health problem and it has a very low survival rate. And, UL16 binding protein 2 (ULBP2) is a new biomarker for PC. We have developed a simple, reliable and inexpensive impedance detection system, which is mainly divided into two parts: immunosensor and readout circuit to detect PC. The ULBP2 immunosensor was fabricated using screen-printed technology. The immunosensor has a sensitivity of 18.5 Ω/Log(pg/mL), with good linearity (R2 = 0.96) and long-term storage stability (retaining 95% activity after 28 days storage). The readout circuit is based on microprocessor to control impedance readout circuit. Evaluations showed that the readout circuit has good accuracy as compared with a commercial impedance analyzer. It is therefore concluded that the designed immunosensor can determine the concentration of ULBP2 antigen accurately and the readout circuit has good accuracy.