由農作物根系分離獲得的蕈狀芽孢桿菌菌株(Bacillus mycoides)經過實驗室、溫室及田間的系列測試，證明具有促進農作物(番茄、萵苣、油菜、高麗菜及菜豆等)之植株生育外，尚且可誘導多種農作物抗拒病蟲害的感染與危害。蕈狀芽孢桿菌促進作物生長與防治病害的主要機轉是：它(1)可纏聚於作物根系及植體內部，誘導農作物產生抗病反應；(2)可分泌植物生長激素(IAA)，促進作物根系發展；(3)可釋放二硫二甲基及生物表面素異構物，破壞植物病原菌的細胞膜。蕈狀芽孢桿菌可研製成生物肥料與生物農藥，用於培育多種農作物的健康種苗與防治蘭花黃葉病、草莓萎凋病、番茄白粉病、胡瓜猝倒病及水稻病害等。蕈狀芽孢桿菌製劑產品的主要追求目標，是為了維護農業生態環境的和諧與生產高品質安全的農產品，藉以確保食品安全。本系列產品不但可提供農作物生育的營養肥份外，尚可誘導農作物產生抗病性，有效降低農田過量施用化學肥料與農藥，避免重金屬與農藥污染環境，有效活化土壤生命力，創造農業的永續發展。

Several beneficial Bacillus mycoides isolates were cultured from crop roots and tested for their abilities to promote plant growth under greenhouse and field conditions. Our results have demonstrated that exogenous application of B. mycoides could enhance growth of various plants including tomato, lettuce, rape, cabbage and asparagus bean, and induce resistance against pests and plant pathogens. Our studies have also found that the mechanisms underlying plant growth promotion and pest resistance are because: 1) B. mycoides could effectively colonize inside root systems and induce plant defense reactions; 2) B. mycoides could produce and secret the plant promoting hormone indole-acetic acid (IAA) that enhances the development of root systems; 3) B. mycoides could produce and secret dimethyl disulfide (DMDS) and biosurfactins that disrupt cell membranes of pathogens. The isolates of B. mycoides have been developed into a biofungicide and a biofertilizer for culturing healthy crop seedlings and controlling a number of diseases in orchid, tomato, strawberry, cucumber and rice. The ultimate goal of developing B. mycoides-related products is to preserve and protect agricultural ecosystems, to produce high-quality agricultural products with less environmental damages, and to increase food safety. The B. mycoides-related products derived from our research will not only promote plant growth by providing necessary nutrients, but also induce plant resistance against a variety of pathogens. Application of the B. mycoides-derived products could potentially reduce the excessive use of chemical fertilizers and pesticides, avoid environmental pollutions due to the use of heavy metals and pesticides, and could improve soil productivity. As such, agricultural production can be sustained for many generations to come.

生物防治、生物肥料、生物農藥

Biocontrol, Biofertilizer, Biofungicide